other:Article Title:
Article Snippet: Antibody Vendors and Cat # Species Working dilution β-catenin BD Transduction Laboratories #610154 mouse IgG 1:200 β-catenin Santa Cruz #sc-7199 rabbit IgG 1:500 AR ThermoFisher #PA1-9005 goat IgG 1:500 MYC Abcam #ab168727 rabbit IgG 1:1000 MYC NovusBio #AF3696 goat IgG 1:500 Ki67 Cell Signaling #9129 rabbit IgG 1:500 pAKT Cell Signaling #9271 rabbit IgG 1:50 CK8 Convance #MMS-162P mouse IgG 1:2000 CK8 Abcam #ab59400 rabbit IgG 1:200 CK14 Abcam #ab7800 mouse IgG 1:200 IGF1R Cell Signaling #9449 rabbit IgG 1:750 pIGF1R Bioss Antibodies #bs-5447R rabbit IgG 1:350 Cyclin D1 Abcam #ab16663 rabbit IgG 1:200 TCF7L2 NovusBio #NBP2-67618 rabbit IgG 1:250 CD44 Santa Cruz #sc-18849 rat IgG 1:200 GFP Cell Signaling #2956 rabbit IgG 1:200 GFP Cell Signaling #2955 mouse IgG 1:200 GFP Abcam #ab13970 chicken IgG 1:2000 IGFBP3 Santa Cruz #sc-365936 mouse IgG 1:500 Vimentin BioLegend #919101 chicken IgG 1:2000 PDGFRβ Abcam #ab32570 rabbit IgG 1:500 P63 BioLegend #W15093 mouse IgG 1:2000 P63 Gene Tex #GTX102425 rabbit IgG 1:1000 pGSK3B Cell Signaling #9331S rabbit IgG 1:50 pGSK3B Proteintech #67558-1-Ig mouse IgG 1:600 TWIST1 gifted by Carlotta Glackin (Beckman Research Institute)1 rabbit IgG 1:800 Biotinylated anti-mouse Vector Laboratories #BA-9200 goat IgG 1:750 Biotinylated anti-rabbit Vector Laboratories #BA-1000 goat IgG 1:750 Biotinylated anti-goat Vector Laboratories #BA-5000 rabbit IgG 1:750 Biotinylated anti-rat Vector Laboratories #BA-9400 goat IgG 1:750 Goat anti-rabbit 488 Invitrogen #A11034 goat IgG 1:500 Goat anti-mouse 488 Invitrogen #A11001 goat IgG 1:500 Goat anti-rabbit 594 Invitrogen #A11012 goat IgG 1:500 Goat anti-mouse 594 Invitrogen #A11005 goat IgG 1:500 Goat anti-chicken 647 Invitrogen #A31571 goat IgG 1:500 Donkey anti-rabbit 488 Invitrogen #A21206 donkey IgG 1:500 Donkey anti-mouse 488 Invitrogen #A21202 donkey IgG 1:500 Donkey anti-rabbit 594 Invitrogen #A21207 donkey IgG 1:500 Donkey anti-mouse 594 Invitrogen #A21203 donkey IgG 1:500 Donkey anti-goat 647 Invitorgen #A21447 donkey IgG 1:500 Supplementary Table 2: List of primers used for qRT-PCR and ChIP-qPCR in this study.
Article Title: ANDROGEN SIGNALING IS ESSENTIAL FOR DEVELOPMENT OF PROSTATE CANCER INITIATED FROM PROSATIC BASAL CELLS
Article Snippet: Antibody Information Antibody Supplier IgG Dilution GFP Molecular Probes #A11122 rabbit IgG (1:1000) GFP Cell Signaling #2955 mouse lgG (1:300) GFP Abcam #ab13970 chicken lgY (1:2000) CK5 Covance #PRB-160P rabbit IgG (1:3000) CK8 Covance #MMS-162P mouse lgG (1:2500) p63 Santa Cruz #sc-8431 mouse lgG (1:200) p63 GeneTex #GTX102425 rabbit IgG (1:1000) β-catenin Santa Cruz#sc-7199 rabbit IgG (1:500) β-catenin BD Transduction Laboratories #610154 mouse lgG (1:1000) Ki67 Cell Signaling #9129 rabbit IgG (1:400) E-cadherin BD Transduction Laboratories #c20820 mouse lgG (1:300) Androgen receptor Santa Cruz #sc-816 rabbit IgG (1:500) Androgen receptor Santa Cruz #sc-7305 mouse lgG (1:200) Synaptophysin Invitrogen #18-0130 rabbit lgG (1:500)
Recombinant:Article Title: Adaptive Fibrogenic Reprogramming of Osteosarcoma Stem Cells Promotes Metastatic Growth.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fibronectin BD Cat# 610077; RRID: AB_2105706 Fibronectin SANTA CRUZ Cat# sc-9068; RRID: AB_2105699 COL-1 SIGMA-ALDRICH Cat# C2456; RRID: AB_476836 S100A4 abcam Cat# ab27957; RRID: AB_2183775 p-Src Cell signaling Cat# 2101; RRID: AB_331697 Src Cell signaling Cat# 2109; RRID: AB_2106059 p-Paxillin Cell signaling Cat# 2541; RRID: AB_2174466 Paxillin Cell signaling Cat# 2542; RRID: AB_10693603 Vimentin SANTA CRUZ Cat# sc-6260; RRID: AB_628437 aSMA SIGMA-ALDRICH Cat# A5228; RRID: AB_262054 Integrin-b3 Cell signaling Cat# 4702; RRID: AB_2296320 Integrin-b3 abcam Cat# ab119992; RRID: AB_10903329 p-CDK2 Cell signaling Cat# 2561; RRID: AB_2078685 GAPDH Cell signaling Cat# 5174; RRID: AB_10622025 p-Cyclin E Cell signaling Cat# 4136; RRID: AB_2071080 HCAM SANTA CRUZ Cat# SC-7946; RRID: AB_2076217 FGF Receptor MILLIPORE Cat# MAB125; RRID: AB_2103537 FGFR1 Cell signaling Cat# 3472; RRID: AB_2103374 FGFR2 Cell signaling Cat# 11835 FGFR3 Cell signaling Cat# 4574; RRID: AB_2246903 FGFR4 Cell signaling Cat# 2894; RRID: AB_2293993 E2F-1 BD Cat# 554213; RRID: AB_395308 SSEA-4 R&D Cat# FAB1435P; RRID: AB_357038 SSEA-4 MILLIPORE Cat# MAB4304; RRID: AB_177629 SSEA-4 R&D Cat# MAB1435; RRID: AB_357704 p27 SANTA CRUZ Cat# sc-528; RRID: AB_632129 p21 Cell signaling Cat# 2947; RRID: AB_823586 Ki-67 EPITOMICS Cat# 2746-1; RRID: AB_2142233 RB BD Cat# 554136; RRID: AB_395259 p-S6K Cell signaling Cat# 9205; RRID: AB_330944 S6K Cell signaling Cat# 9202; RRID: AB_331676 p-AKT Cell signaling Cat# 2965; RRID: AB_2255933 AKT Cell signaling Cat# 4685; RRID: AB_2225340 p-ERK Cell signaling Cat# 4370; RRID: AB_2315112 ERK Cell signaling Cat# 4695; RRID: AB_390779 p-S6 Cell signaling Cat# 2211; RRID: AB_331679 S6 Cell signaling Cat# 2317; RRID: AB_2238583 Oct-3/4 R&D Cat# MAB1759; RRID: AB_2167713 Nanog Cell signaling Cat# 4893; RRID: AB_10548762 p-Smad3 Cell signaling Cat# 9520; RRID: AB_2193207 FITC-Ki67 BD Cat# 556026; RRIA: AB_396302 APC-BrdU BD Cat# 556028; RRID: AB_396304 Alexa Fluor 488 Jackson ImmunoResarch Cat# 711-545-152; RRID: AB_2313584 (Continued on next page) e1 Cell Reports 24, 1266–1277.e1–e5, July 31, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cy3 Jackson ImmunoResarch Cat# 715-165-150; RRID: AB_2340813 Anti-b-Actin SIGMA-ALDRICH Cat# A1978; RRID: AB_476692 Anti-Rabbit IgG Peroxidase Conjugate Calbiochem Cat# 401315 Anti-Mouse IgG Peroxidase Conjugate Calbiochem Cat# 401215 GFP Cell signaling Cat# 2956; RRID: AB_1196615 Chemicals, Peptides, and Recombinant Proteins N-2 R&D Cat# AR003 FGF R&D Cat# 233-FB EGF R&D Cat# 236-EG IGF-1 R&D Cat# 291-G1 PDGF-BB R&D Cat# 220-BB LIF MILLIPORE Cat# LIF1005 TGF-b R&D Cat# 240-B B27 GIBCO Cat# 17504 activin A R&D Cat# 338-AC SU5402 Calbiochem Cat# 572630 RAD001 Novartis Pharma N/A LY294002 Calbiochem Cat# 440202 Triton X-100 SIGMA-ALDRICH Cat# X-100 D-luciferin, Potassium Salt Sciencelight Cat# 201105 DMSO SIGMA-ALDRICH Cat# D2650 Doxycycline Clontech Cat# 631311 Puromycin Clontech Cat# 631305 Trizol Invitrogen Cat#15596018 DMEM Invitrogen Cat# 11995 RPMI 1640 Invitrogen Cat# 11875 Trypsin Invitrogen Cat# 25200-072 OPTI-MEM Invitrogen Cat# 31985-070 Fetal bovine serum Invitrogen Cat# 10099-141 Nintedanib Selleck Cat# S1010 Pirfenidone Selleck Cat# S2907 Critical Commercial Assays ImmobilonTM Western Chemiluminescent HRP Substrate MILLIPORE Cat# WBKLS0500 Dynabeads M-280 Sheep Anti-Mouse IgG Invitrogen Cat# 11201D MOUNTING MEDIUM VECTOR Cat# H-1200 Apoptosis Detection Kit BD Cat# 556547 Anti-Human Ki-67 Set BD Cat# 556026 Senescence Detection Kit abcam Cat# Ab65351-250 Anti-BrdU Set BD Cat# 556028 SYBR Premix ExTaq kit Takara Cat#RR420A Deposited Data Raw gene microarray data This paper GEO: GSE85537 Experimental Models: Cell Lines Human osteosarcoma cell line MG63 Shanghai Institute of Hematology MG63 Human osteosarcoma cell line Well5 Shanghai Institute of Hematology Well5 Human osteosarcoma cell line Saos2 Shanghai Institute of Hematology Saos2 Human osteosarcoma cell line U2OS Shanghai Institute of Hematology U2OS Human breast cancer cell line MCF-7 Shanghai Institute of Hematology MCF-7 (Continued on next page) Cell Reports 24, 1266–1277.e1–e5, July 31, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human pancreatic cancer cell line PANC-1 Shanghai Institute of Hematology PANC-1 Human liver cancer cell line HepG2 Shanghai Institute of Hematology HepG2 Human liver cancer cell line 7402 Shanghai Institute of Hematology 7402 Human pancreatic cancer cell line SW1990 Shanghai Institute of Hematology SW1990 Experimental Models: Organisms/Strains NOD/SCID mice Shanghai SLAC Laboratory NOD/SCID Oligonucleotides sg1: AGCCCGGGCCAATCGGCGCG Shanghai Genechem Cat# V20161209006 sg2: CAGTCCCCCGTGACGTCACC sg3: CGGGGCGGGGACAGCCCGGC FN1-shRNAs Shanghai Genechem Cat# GCIL922416 #1 50-CGTGGTTGTATCAGGACTTAT-30 #2 50-GCCTGCTCCAAGAATTGGTTT-30 #3 50-CCCTACACAGTTTCCCATTAT-30 FGFR2-shRNAs Shanghai Genechem Cat# V20161110022 #1 50-CAACCTCTCGAACAGTATT-30 #2 50-CAACAATAGGACAGTGCTT-30 #3 50-CACCAACCAAATACCAAAT-30 FN primer Sangon Biotech N/A 50-CCA CCC CCA TAA GGC ATA GG-30 and 50-GTA GGG GTC AAA GCA CGA GTC ATC-30 Software and Algorithms FlowJo software TreeStar N/A Image Reader LAS-4000 FUJIFILM N/A Leica TCS SP8 Leica https://www.leica-microsystems.com/ products/confocal-microscopes/details/ product/leica-tcs-sp8/ GraphPad Prism GraphPad Software http://www.graphpad.com/prism/ Excel 2010 Microsoft N/A Summit software Beckman coulter http://www.beckmancoulter.cn/ls- discovery/flow.aspx Other Normal Donkey Serum Jackson ImmunoResarch Cat# 017-000-121
Western Blot:Article Title: Adaptive Fibrogenic Reprogramming of Osteosarcoma Stem Cells Promotes Metastatic Growth.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fibronectin BD Cat# 610077; RRID: AB_2105706 Fibronectin SANTA CRUZ Cat# sc-9068; RRID: AB_2105699 COL-1 SIGMA-ALDRICH Cat# C2456; RRID: AB_476836 S100A4 abcam Cat# ab27957; RRID: AB_2183775 p-Src Cell signaling Cat# 2101; RRID: AB_331697 Src Cell signaling Cat# 2109; RRID: AB_2106059 p-Paxillin Cell signaling Cat# 2541; RRID: AB_2174466 Paxillin Cell signaling Cat# 2542; RRID: AB_10693603 Vimentin SANTA CRUZ Cat# sc-6260; RRID: AB_628437 aSMA SIGMA-ALDRICH Cat# A5228; RRID: AB_262054 Integrin-b3 Cell signaling Cat# 4702; RRID: AB_2296320 Integrin-b3 abcam Cat# ab119992; RRID: AB_10903329 p-CDK2 Cell signaling Cat# 2561; RRID: AB_2078685 GAPDH Cell signaling Cat# 5174; RRID: AB_10622025 p-Cyclin E Cell signaling Cat# 4136; RRID: AB_2071080 HCAM SANTA CRUZ Cat# SC-7946; RRID: AB_2076217 FGF Receptor MILLIPORE Cat# MAB125; RRID: AB_2103537 FGFR1 Cell signaling Cat# 3472; RRID: AB_2103374 FGFR2 Cell signaling Cat# 11835 FGFR3 Cell signaling Cat# 4574; RRID: AB_2246903 FGFR4 Cell signaling Cat# 2894; RRID: AB_2293993 E2F-1 BD Cat# 554213; RRID: AB_395308 SSEA-4 R&D Cat# FAB1435P; RRID: AB_357038 SSEA-4 MILLIPORE Cat# MAB4304; RRID: AB_177629 SSEA-4 R&D Cat# MAB1435; RRID: AB_357704 p27 SANTA CRUZ Cat# sc-528; RRID: AB_632129 p21 Cell signaling Cat# 2947; RRID: AB_823586 Ki-67 EPITOMICS Cat# 2746-1; RRID: AB_2142233 RB BD Cat# 554136; RRID: AB_395259 p-S6K Cell signaling Cat# 9205; RRID: AB_330944 S6K Cell signaling Cat# 9202; RRID: AB_331676 p-AKT Cell signaling Cat# 2965; RRID: AB_2255933 AKT Cell signaling Cat# 4685; RRID: AB_2225340 p-ERK Cell signaling Cat# 4370; RRID: AB_2315112 ERK Cell signaling Cat# 4695; RRID: AB_390779 p-S6 Cell signaling Cat# 2211; RRID: AB_331679 S6 Cell signaling Cat# 2317; RRID: AB_2238583 Oct-3/4 R&D Cat# MAB1759; RRID: AB_2167713 Nanog Cell signaling Cat# 4893; RRID: AB_10548762 p-Smad3 Cell signaling Cat# 9520; RRID: AB_2193207 FITC-Ki67 BD Cat# 556026; RRIA: AB_396302 APC-BrdU BD Cat# 556028; RRID: AB_396304 Alexa Fluor 488 Jackson ImmunoResarch Cat# 711-545-152; RRID: AB_2313584 (Continued on next page) e1 Cell Reports 24, 1266–1277.e1–e5, July 31, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cy3 Jackson ImmunoResarch Cat# 715-165-150; RRID: AB_2340813 Anti-b-Actin SIGMA-ALDRICH Cat# A1978; RRID: AB_476692 Anti-Rabbit IgG Peroxidase Conjugate Calbiochem Cat# 401315 Anti-Mouse IgG Peroxidase Conjugate Calbiochem Cat# 401215 GFP Cell signaling Cat# 2956; RRID: AB_1196615 Chemicals, Peptides, and Recombinant Proteins N-2 R&D Cat# AR003 FGF R&D Cat# 233-FB EGF R&D Cat# 236-EG IGF-1 R&D Cat# 291-G1 PDGF-BB R&D Cat# 220-BB LIF MILLIPORE Cat# LIF1005 TGF-b R&D Cat# 240-B B27 GIBCO Cat# 17504 activin A R&D Cat# 338-AC SU5402 Calbiochem Cat# 572630 RAD001 Novartis Pharma N/A LY294002 Calbiochem Cat# 440202 Triton X-100 SIGMA-ALDRICH Cat# X-100 D-luciferin, Potassium Salt Sciencelight Cat# 201105 DMSO SIGMA-ALDRICH Cat# D2650 Doxycycline Clontech Cat# 631311 Puromycin Clontech Cat# 631305 Trizol Invitrogen Cat#15596018 DMEM Invitrogen Cat# 11995 RPMI 1640 Invitrogen Cat# 11875 Trypsin Invitrogen Cat# 25200-072 OPTI-MEM Invitrogen Cat# 31985-070 Fetal bovine serum Invitrogen Cat# 10099-141 Nintedanib Selleck Cat# S1010 Pirfenidone Selleck Cat# S2907 Critical Commercial Assays ImmobilonTM Western Chemiluminescent HRP Substrate MILLIPORE Cat# WBKLS0500 Dynabeads M-280 Sheep Anti-Mouse IgG Invitrogen Cat# 11201D MOUNTING MEDIUM VECTOR Cat# H-1200 Apoptosis Detection Kit BD Cat# 556547 Anti-Human Ki-67 Set BD Cat# 556026 Senescence Detection Kit abcam Cat# Ab65351-250 Anti-BrdU Set BD Cat# 556028 SYBR Premix ExTaq kit Takara Cat#RR420A Deposited Data Raw gene microarray data This paper GEO: GSE85537 Experimental Models: Cell Lines Human osteosarcoma cell line MG63 Shanghai Institute of Hematology MG63 Human osteosarcoma cell line Well5 Shanghai Institute of Hematology Well5 Human osteosarcoma cell line Saos2 Shanghai Institute of Hematology Saos2 Human osteosarcoma cell line U2OS Shanghai Institute of Hematology U2OS Human breast cancer cell line MCF-7 Shanghai Institute of Hematology MCF-7 (Continued on next page) Cell Reports 24, 1266–1277.e1–e5, July 31, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human pancreatic cancer cell line PANC-1 Shanghai Institute of Hematology PANC-1 Human liver cancer cell line HepG2 Shanghai Institute of Hematology HepG2 Human liver cancer cell line 7402 Shanghai Institute of Hematology 7402 Human pancreatic cancer cell line SW1990 Shanghai Institute of Hematology SW1990 Experimental Models: Organisms/Strains NOD/SCID mice Shanghai SLAC Laboratory NOD/SCID Oligonucleotides sg1: AGCCCGGGCCAATCGGCGCG Shanghai Genechem Cat# V20161209006 sg2: CAGTCCCCCGTGACGTCACC sg3: CGGGGCGGGGACAGCCCGGC FN1-shRNAs Shanghai Genechem Cat# GCIL922416 #1 50-CGTGGTTGTATCAGGACTTAT-30 #2 50-GCCTGCTCCAAGAATTGGTTT-30 #3 50-CCCTACACAGTTTCCCATTAT-30 FGFR2-shRNAs Shanghai Genechem Cat# V20161110022 #1 50-CAACCTCTCGAACAGTATT-30 #2 50-CAACAATAGGACAGTGCTT-30 #3 50-CACCAACCAAATACCAAAT-30 FN primer Sangon Biotech N/A 50-CCA CCC CCA TAA GGC ATA GG-30 and 50-GTA GGG GTC AAA GCA CGA GTC ATC-30 Software and Algorithms FlowJo software TreeStar N/A Image Reader LAS-4000 FUJIFILM N/A Leica TCS SP8 Leica https://www.leica-microsystems.com/ products/confocal-microscopes/details/ product/leica-tcs-sp8/ GraphPad Prism GraphPad Software http://www.graphpad.com/prism/ Excel 2010 Microsoft N/A Summit software Beckman coulter http://www.beckmancoulter.cn/ls- discovery/flow.aspx Other Normal Donkey Serum Jackson ImmunoResarch Cat# 017-000-121
Microarray:Article Title: Adaptive Fibrogenic Reprogramming of Osteosarcoma Stem Cells Promotes Metastatic Growth.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fibronectin BD Cat# 610077; RRID: AB_2105706 Fibronectin SANTA CRUZ Cat# sc-9068; RRID: AB_2105699 COL-1 SIGMA-ALDRICH Cat# C2456; RRID: AB_476836 S100A4 abcam Cat# ab27957; RRID: AB_2183775 p-Src Cell signaling Cat# 2101; RRID: AB_331697 Src Cell signaling Cat# 2109; RRID: AB_2106059 p-Paxillin Cell signaling Cat# 2541; RRID: AB_2174466 Paxillin Cell signaling Cat# 2542; RRID: AB_10693603 Vimentin SANTA CRUZ Cat# sc-6260; RRID: AB_628437 aSMA SIGMA-ALDRICH Cat# A5228; RRID: AB_262054 Integrin-b3 Cell signaling Cat# 4702; RRID: AB_2296320 Integrin-b3 abcam Cat# ab119992; RRID: AB_10903329 p-CDK2 Cell signaling Cat# 2561; RRID: AB_2078685 GAPDH Cell signaling Cat# 5174; RRID: AB_10622025 p-Cyclin E Cell signaling Cat# 4136; RRID: AB_2071080 HCAM SANTA CRUZ Cat# SC-7946; RRID: AB_2076217 FGF Receptor MILLIPORE Cat# MAB125; RRID: AB_2103537 FGFR1 Cell signaling Cat# 3472; RRID: AB_2103374 FGFR2 Cell signaling Cat# 11835 FGFR3 Cell signaling Cat# 4574; RRID: AB_2246903 FGFR4 Cell signaling Cat# 2894; RRID: AB_2293993 E2F-1 BD Cat# 554213; RRID: AB_395308 SSEA-4 R&D Cat# FAB1435P; RRID: AB_357038 SSEA-4 MILLIPORE Cat# MAB4304; RRID: AB_177629 SSEA-4 R&D Cat# MAB1435; RRID: AB_357704 p27 SANTA CRUZ Cat# sc-528; RRID: AB_632129 p21 Cell signaling Cat# 2947; RRID: AB_823586 Ki-67 EPITOMICS Cat# 2746-1; RRID: AB_2142233 RB BD Cat# 554136; RRID: AB_395259 p-S6K Cell signaling Cat# 9205; RRID: AB_330944 S6K Cell signaling Cat# 9202; RRID: AB_331676 p-AKT Cell signaling Cat# 2965; RRID: AB_2255933 AKT Cell signaling Cat# 4685; RRID: AB_2225340 p-ERK Cell signaling Cat# 4370; RRID: AB_2315112 ERK Cell signaling Cat# 4695; RRID: AB_390779 p-S6 Cell signaling Cat# 2211; RRID: AB_331679 S6 Cell signaling Cat# 2317; RRID: AB_2238583 Oct-3/4 R&D Cat# MAB1759; RRID: AB_2167713 Nanog Cell signaling Cat# 4893; RRID: AB_10548762 p-Smad3 Cell signaling Cat# 9520; RRID: AB_2193207 FITC-Ki67 BD Cat# 556026; RRIA: AB_396302 APC-BrdU BD Cat# 556028; RRID: AB_396304 Alexa Fluor 488 Jackson ImmunoResarch Cat# 711-545-152; RRID: AB_2313584 (Continued on next page) e1 Cell Reports 24, 1266–1277.e1–e5, July 31, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cy3 Jackson ImmunoResarch Cat# 715-165-150; RRID: AB_2340813 Anti-b-Actin SIGMA-ALDRICH Cat# A1978; RRID: AB_476692 Anti-Rabbit IgG Peroxidase Conjugate Calbiochem Cat# 401315 Anti-Mouse IgG Peroxidase Conjugate Calbiochem Cat# 401215 GFP Cell signaling Cat# 2956; RRID: AB_1196615 Chemicals, Peptides, and Recombinant Proteins N-2 R&D Cat# AR003 FGF R&D Cat# 233-FB EGF R&D Cat# 236-EG IGF-1 R&D Cat# 291-G1 PDGF-BB R&D Cat# 220-BB LIF MILLIPORE Cat# LIF1005 TGF-b R&D Cat# 240-B B27 GIBCO Cat# 17504 activin A R&D Cat# 338-AC SU5402 Calbiochem Cat# 572630 RAD001 Novartis Pharma N/A LY294002 Calbiochem Cat# 440202 Triton X-100 SIGMA-ALDRICH Cat# X-100 D-luciferin, Potassium Salt Sciencelight Cat# 201105 DMSO SIGMA-ALDRICH Cat# D2650 Doxycycline Clontech Cat# 631311 Puromycin Clontech Cat# 631305 Trizol Invitrogen Cat#15596018 DMEM Invitrogen Cat# 11995 RPMI 1640 Invitrogen Cat# 11875 Trypsin Invitrogen Cat# 25200-072 OPTI-MEM Invitrogen Cat# 31985-070 Fetal bovine serum Invitrogen Cat# 10099-141 Nintedanib Selleck Cat# S1010 Pirfenidone Selleck Cat# S2907 Critical Commercial Assays ImmobilonTM Western Chemiluminescent HRP Substrate MILLIPORE Cat# WBKLS0500 Dynabeads M-280 Sheep Anti-Mouse IgG Invitrogen Cat# 11201D MOUNTING MEDIUM VECTOR Cat# H-1200 Apoptosis Detection Kit BD Cat# 556547 Anti-Human Ki-67 Set BD Cat# 556026 Senescence Detection Kit abcam Cat# Ab65351-250 Anti-BrdU Set BD Cat# 556028 SYBR Premix ExTaq kit Takara Cat#RR420A Deposited Data Raw gene microarray data This paper GEO: GSE85537 Experimental Models: Cell Lines Human osteosarcoma cell line MG63 Shanghai Institute of Hematology MG63 Human osteosarcoma cell line Well5 Shanghai Institute of Hematology Well5 Human osteosarcoma cell line Saos2 Shanghai Institute of Hematology Saos2 Human osteosarcoma cell line U2OS Shanghai Institute of Hematology U2OS Human breast cancer cell line MCF-7 Shanghai Institute of Hematology MCF-7 (Continued on next page) Cell Reports 24, 1266–1277.e1–e5, July 31, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human pancreatic cancer cell line PANC-1 Shanghai Institute of Hematology PANC-1 Human liver cancer cell line HepG2 Shanghai Institute of Hematology HepG2 Human liver cancer cell line 7402 Shanghai Institute of Hematology 7402 Human pancreatic cancer cell line SW1990 Shanghai Institute of Hematology SW1990 Experimental Models: Organisms/Strains NOD/SCID mice Shanghai SLAC Laboratory NOD/SCID Oligonucleotides sg1: AGCCCGGGCCAATCGGCGCG Shanghai Genechem Cat# V20161209006 sg2: CAGTCCCCCGTGACGTCACC sg3: CGGGGCGGGGACAGCCCGGC FN1-shRNAs Shanghai Genechem Cat# GCIL922416 #1 50-CGTGGTTGTATCAGGACTTAT-30 #2 50-GCCTGCTCCAAGAATTGGTTT-30 #3 50-CCCTACACAGTTTCCCATTAT-30 FGFR2-shRNAs Shanghai Genechem Cat# V20161110022 #1 50-CAACCTCTCGAACAGTATT-30 #2 50-CAACAATAGGACAGTGCTT-30 #3 50-CACCAACCAAATACCAAAT-30 FN primer Sangon Biotech N/A 50-CCA CCC CCA TAA GGC ATA GG-30 and 50-GTA GGG GTC AAA GCA CGA GTC ATC-30 Software and Algorithms FlowJo software TreeStar N/A Image Reader LAS-4000 FUJIFILM N/A Leica TCS SP8 Leica https://www.leica-microsystems.com/ products/confocal-microscopes/details/ product/leica-tcs-sp8/ GraphPad Prism GraphPad Software http://www.graphpad.com/prism/ Excel 2010 Microsoft N/A Summit software Beckman coulter http://www.beckmancoulter.cn/ls- discovery/flow.aspx Other Normal Donkey Serum Jackson ImmunoResarch Cat# 017-000-121
Chromatin Immunoprecipitation:Article Title: Aberrant androgen action in prostatic progenitor cells induces oncogenesis and tumor development through IGF1 and Wnt axes
Article Snippet: .. Antibody Vendors and Cat # Species Working dilution GFP Cell Signaling #2956 rabbit IgG 1:200 GFP Cell Signaling #2955 mouse IgG 1:200 GFP Abcam #ab13970 chicken IgG 1:2000 OSR1 Biorbyt #orb162212 rabbit IgG 1:300 AR ThermoFisher #PA1-9005 goat IgG 1:500 CK8 Convance #MMS-162P mouse IgG 1:2000 CK8 Abcam #ab59400 Rabbit IgG 1:1000 CK5 Convance #PRB-160P rabbit IgG 1:2400 P63 Biolegend #687202 mouse IgG 1:500 Vimentin BioLegend #919101 chicken IgG 1:2000 SMA Sigma Aldrich # A5228 mouse IgG 1:2000 hAR Santa Cruz #sc-7305 mouse IgG 1:100 CK14 Abcam #ab7800 mouse IgG 1:200 IGF1R Cell Signaling #9449 rabbit IgG 1:750 pIGF1R Bioss Antibodies #bs-5447R rabbit IgG 1:350 β-catenin BD Transduction Laboratories #610154 mouse IgG 1:200 β-catenin Santa Cruz #sc-7199 rabbit IgG 1:500 MYC Abcam #ab168727 rabbit IgG 1:1000 TCF4 NovusBio #NBP2-67618 rabbit IgG 1:250 Cyclin D1 Abcam #ab16663 rabbit IgG 1:200 AXIN2 Abcam #ab32197 rabbit IgG 1:2000 LGR5 Abcam #ab219107 rabbit IgG 1:500 pAKT Cell Signaling #9271 rabbit IgG 1:50 pGSK3 Cell Signaling #9331 rabbit IgG 1:200 p-ERK1/2 Cell Signaling #4370 rabbit IgG 1:100 Biotinylated anti-mouse Vector Laboratories #BA-9200 goat IgG 1:750 Biotinylated anti-rabbit Vector Laboratories #BA-1000 goat IgG 1:750 Biotinylated anti-goat Vector Laboratories #BA-5000 rabbit IgG 1:750 Biotinylated anti-rat Vector Laboratories #BA-9400 goat IgG 1:750 Goat anti-rabbit 488 Invitrogen #A11034 goat IgG 1:500 Goat anti-mouse 488 Invitrogen #A11001 goat IgG 1:500 Goat anti-rabbit 594 Invitrogen #A11012 goat IgG 1:500 Goat anti-mouse 594 Invitrogen #A11005 goat IgG 1:500 Goat anti-chicken 647 Invitrogen #A31571 goat IgG 1:500 Donkey anti-rabbit 488 Invitrogen #A21206 donkey IgG 1:500 Donkey anti-mouse 488 Invitrogen #A21202 donkey IgG 1:500 Donkey anti-rabbit 594 Invitrogen #A21207 donkey IgG 1:500 Donkey anti-mouse 594 Invitrogen #A21203 donkey IgG 1:500 Donkey anti-goat 647 Invitorgen #A21447 donkey IgG 1:500 hAR for ChIP-seq Santa Cruz #sc-7305X mouse IgG 1:100 ..
Immunohistochemistry:Article Title:
Article Snippet: .. All antibodies for immunohistochemistry and immonfluorescence used were from commercial sources: β-catenin BD Transduction Laboratories #610154 mouse IgG 1:200, β-catenin Santa Cruz #sc-7199 rabbit IgG 1:500, AR ThermoFisher #PA1-9005 goat IgG 1:500, MYC Abcam #ab168727 rabbit IgG 1:1000, MYC NovusBio #AF3696 goat IgG 1:500, Ki67 Cell Signaling #9129 rabbit IgG 1:500, pAKT Cell Signaling #9271 rabbit IgG 1:50, CK8 Convance #MMS-162P mouse IgG 1:2000, CK14 Abcam #ab7800 mouse IgG 1:200, IGF1R Cell Signaling #9750 rabbit IgG 1:750, pIGF1R Bioss Antibodies #bs-5447R rabbit IgG 1:350, Cyclin D1 Abcam #ab16663 rabbit IgG 1:200, TCF7L2 NovusBio #NBP2-67618 rabbit IgG 1:250, CD44 Santa Cruz #sc-18849 rat IgG 1:200, GFP Cell Signaling #2956 rabbit IgG 1:200, GFP Cell Signaling #2955 mouse IgG 1:200, GFP Abcam #ab13970 chicken IgG 1:2000, IGFBP3 Santa Cruz #sc-365936 mouse IgG 1:500, Vimentin BioLegend #919101 chicken IgG 1:2000, PDGFRβ Abcam #ab32570 rabbit IgG 1:500, Biotinylated anti-mouse Vector Laboratories #BA-9200 goat IgG 1:750, Biotinylated anti-rabbit Vector Laboratories #BA-1000 goat IgG 1:750, Biotinylated anti-goat Vector Laboratories #BA-5000 rabbit IgG 1:750, Biotinylated anti-rat Vector Laboratories #BA-9400 goat IgG 1:750, Goat anti-rabbit 488 Invitrogen #A11034 goat IgG 1:500, Goat anti-mouse 488 Invitrogen #A11001 goat IgG 1:500, Goat anti-rabbit 594 Invitrogen #A11012 goat IgG 1:500, Goat anti-mouse 594 Invitrogen #A11005 goat IgG 1:500, Goat anti-chicken 647 Invitrogen #A21449 goat IgG 1:500, Donkey anti-rabbit 488 Invitrogen #A21206 donkey IgG 1:500, Donkey anti-mouse 488 Invitrogen #A21202 donkey IgG 1:500, Donkey anti-rabbit 594 Invitrogen #A21207 donkey IgG 1:500, Donkey anti-mouse 594 Invitrogen #A21203 donkey IgG 1:500, Donkey anti-goat 647 Invitrogen #A21447 donkey IgG 1:500. .. CK8 Abcam #ab59400 rabbit IgG 1:200.
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